期刊
JOURNAL OF CELL SCIENCE
卷 122, 期 16, 页码 2980-2988出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.041970
关键词
Actin isoforms; Actin network; Cytoskeleton
类别
资金
- Swiss National Science Foundation [3100A0-109879, PMPDA-102408]
- EMBO fellowship
- Russian Foundation of Basic Investigation [06-04-49214a]
- Faculty of Medicine, University of Geneva
Using newly generated monoclonal antibodies, we have compared the distribution of beta- and gamma-cytoplasmic actin in fibroblastic and epithelial cells, in which they play crucial roles during various key cellular processes. Whereas beta-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction,gamma-actin displays a more versatile organization, according to cell activities. In moving cells, gamma-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility; in stationary cells, gamma-actin is also recruited into stress fibers. beta-actin-depleted cells become highly spread, display broad protrusions and reduce their stress-fiber content; by contrast, gamma-actin-depleted cells acquire a contractile phenotype with thick actin bundles and shrinked lamellar and lamellipodial structures. Moreover, beta- and gamma-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion. Our results reveal new aspects of beta- and gamma-actin organization that support their functional diversity.
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