期刊
JOURNAL OF CELL SCIENCE
卷 122, 期 20, 页码 3619-3626出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.054437
关键词
Stress response; mRNA storage; mRNA degradation
类别
资金
- Ministsre de l'Enseignement Superieur
- Centre National de la Recherche Scientifique
- Association pour la Recherche contre le Cancer
- Agence Nationale pour la Recherche
Stress granules are cytoplasmic ribonucleoprotein granules formed following various stresses that inhibit translation. They are thought to help protecting untranslated mRNAs until stress relief. Stress granules are frequently seen adjacent to P-bodies, which are involved in mRNA degradation and storage. We have previously shown in live cells that stress granule assembly often takes place in the vicinity of pre-existing P-bodies, suggesting that these two compartments are structurally related. Here we provide the first ultrastructural characterization of stress granules in eukaryotic cells by electron microscopy. Stress granules resulting from oxidative stress, heat-shock or protein overexpression are loosely organised fibrillo-granular aggregates of a moderate electron density, whereas P-bodies are denser and fibrillar. By in situ hybridization at the electron microscopic level, we show that stress granules are enriched in poly(A)(+) mRNAs, although these represent a minor fraction of the cellular mRNAs. Finally, we show that, despite close contact with P-bodies, both domains remain structurally distinct and do not interdigitate.
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