期刊
JOURNAL OF CELL SCIENCE
卷 122, 期 12, 页码 2149-2159出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.046706
关键词
CDK2; Chromosome synapsis; Meiosis; Meiotic recombination; Mouse; Sex body
类别
资金
- Ministerio de Educacion y Ciencia [BFU2005-05668-C03, BFU2006-06655, BFU2006-04406]
- Comunidad de Madrid [GR/SAL/0206/2004, P-BIO-0189-2006]
Cyclin-dependent kinase 2 (CDK2) was assumed to be essential in the mammalian cell cycle both at the G1-S transition and throughout the S phase. Interestingly, ablation of Cdk2 in mice does not have substantial consequences for embryonic or postnatal development, but both males and females are infertile. In the present study, we have analysed the meiotic alterations leading to infertility in Cdk2-/- male mice. We have studied the distribution and dynamics of several proteins related to meiosis progression, such as synaptonemal complex proteins, cohesin complexes, and centromere-, telomere- and recombination-related proteins. Cdk2-/- spermatocytes show an incomplete chromosome pairing, an extensive non-homologous synapsis and arrest at a pachytene-like stage with unrepaired programmed double-strand breaks. In these spermatocytes, some telomeres do not attach to the nuclear envelope, and sex chromosomes do not form a sex body. Our data demonstrate an unpredicted participation of CDK2 in the accurate pairing and recombination between homologues during mammalian meiosis.
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