期刊
JOURNAL OF CELL SCIENCE
卷 122, 期 20, 页码 3749-3758出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.050625
关键词
AtRab1b; ARA5; Ypt1; Rab GTPases; pOp6/LhGR
类别
资金
- BBSRC [BBS/B/03904]
- BBSRC CASE
- HFSP
- Swiss National Science Foundation
- HHMI
- USDA
- NIH
- Michele Bellucci (Istituto di Genetica Vegetale-Sezione di Perugia, Perugia, Italy)
- Paul Dupree (University of Cambridge, Cambridge, UK)
- Biotechnology and Biological Sciences Research Council [BBS/B/03904, REI20537] Funding Source: researchfish
GTPases of the Rab1 subclass are essential for membrane traffic between the endoplasmic reticulum (ER) and Golgi complex in animals, fungi and plants. Rab1-related proteins in higher plants are unusual because sequence comparisons divide them into two putative subclasses, Rab-D1 and Rab-D2, that are conserved in monocots and dicots. We tested the hypothesis that the Rab-D1 and Rab-D2 proteins of Arabidopsis represent functionally distinct groups. RAB-D1 and RAB-D2a each targeted fluorescent proteins to the same punctate structures associated with the Golgi stacks and trans-Golgi-network. Dominant-inhibitory N121I mutants of each protein inhibited traffic of diverse cargo proteins at the ER but they appeared to act via distinct biochemical pathways as biosynthetic traffic in cells expressing either of the N121I mutants could be restored by coexpressing the wild-type form of the same subclass but not the other subclass. The same interaction was observed in transgenic seedlings expressing RAB-D1 [N121I]. Insertional mutants confirmed that the three Arabidopsis Rab-D2 genes were extensively redundant and collectively performed an essential function that could not be provided by RAB-D1, which was nonessential. However, plants lacking RAB-D1, RAB-D2b and RAB-D2c were short and bushy with low fertility, indicating that the Rab-D1 and Rab-D2 subclasses have overlapping functions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据