期刊
JOURNAL OF CELL SCIENCE
卷 122, 期 6, 页码 822-833出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.042754
关键词
C. elegans; Ceramide; Glycosphingolipids; Glycosyltransferase; Intestine
类别
资金
- Royal Society
- Medical Research Council
- Cancer Research UK
- NIH National Center for Research Resources (NCRR)
- Medical Research Council [MC_U122663296] Funding Source: researchfish
- MRC [MC_U122663296] Funding Source: UKRI
Glycosphingolipids (GSLs) are glycosylated derivatives of ceramide in the lipid bilayer. Their ubiquitous distribution and complexity suggest that they have important functions, but what these are in vivo is still poorly understood. Here, we characterize the phenotype of Caenorhabditis elegans mutants with essentially no GSLs. The C. elegans genome encodes three ceramide glucosyltransferase (CGT) genes, which encode enzymes required for GSL biosynthesis. Animals lacking CGT do not synthesize GSLs, arrest growth at the first larval stage, and display defects in a subset of cells in their digestive tract; these defects impair larval feeding, resulting in a starvation-induced growth arrest. Restoring CGT function in these digestive tract cells - but not in a variety of other tissues - is sufficient to rescue the phenotypes associated with loss of CGT function. These unexpected findings suggest that GSLs are dispensable in most C. elegans cells, including those of the nervous system.
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