期刊
JOURNAL OF CELL SCIENCE
卷 122, 期 22, 页码 4186-4194出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.058420
关键词
Calcium-activated potassium channel; Trafficking; Endocytosis; Whole-cell recording; Hippocampal neurons
类别
资金
- Medical Research Council (UK)
- MRC [G0601813] Funding Source: UKRI
- Medical Research Council [G0601813] Funding Source: researchfish
Trafficking motifs present in the intracellular regions of ion channels affect their subcellular location within neurons. The mechanisms that control trafficking to dendrites of central neurons have been identified, but it is not fully understood how channels are localized to the soma. We have now identified a motif within the calcium-activated potassium channel K(Ca)2.1 (SK1) that results in somatic localization. Transfection of hippocampal neurons with K(Ca)2.1 subunits causes expression of functional channels in only the soma and proximal processes. By contrast, expressed K(Ca)2.3 subunits are located throughout the processes of transfected neurons. Point mutation of K(Ca)2.1 within this novel motif to mimic a sequence present in the C-terminus of K(Ca)2.3 causes expression of K(Ca)2.1 subunits throughout the processes. We also demonstrate that blocking of clathrin-mediated endocytosis causes K(Ca)2.1 subunit expression to mimic that of the mutated subunit. The role of this novel motif is therefore not to directly target trafficking of the channel to subcellular compartments, but to regulate channel location by subjecting it to rapid clathrin-mediated endocytosis.
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