期刊
JOURNAL OF CELL SCIENCE
卷 121, 期 5, 页码 571-577出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.023234
关键词
chromatin condensation; chromosome territory; colinearity; Hox; nuclear organisation
类别
资金
- Medical Research Council [MC_U127527202, U.1275.01.006(61090)] Funding Source: Medline
- Medical Research Council [MC_U127527202] Funding Source: researchfish
- MRC [MC_U127527202] Funding Source: UKRI
The extent to which the nuclear organisation of a gene impacts on its ability to be expressed, or whether nuclear organisation merely reflects gene expression states, remains an important but unresolved issue. A model system that has been instrumental in investigating this question utilises the murine Hox gene clusters encoding homeobox-containing proteins. Nuclear reorganisation and chromatin decondensation, initiated towards the 3' end of the clusters, accompanies activation of Hox genes in both differentiation and development, and might be linked to mechanisms underlying colinearity. To investigate this, and to delineate the cis-acting elements involved, here we analyse the nuclear behaviour of a 3' Hoxb1 transgene transposed to the 5' end of the Hoxd cluster. We show that this transgene contains the cis-acting elements sufficient to initiate ectopic local nuclear reorganisation and chromatin decondensation and to break Hoxd colinearity in the primitive streak region of the early embryo. Significantly, in rhombomere 4, the transgene is able to induce attenuated nuclear reorganisation and decondensation of Hoxd even though there is no detectable expression of the transgene at this site. This shows that reorganisation of chromosome territories and chromatin decondensation can be uncoupled from transcription itself and suggests that they can therefore operate upstream of gene expression.
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