期刊
JOURNAL OF CELL SCIENCE
卷 121, 期 12, 页码 1999-2007出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.027250
关键词
bromo-uridine triphosphate; electron microscopy; electron spectroscopic imaging; transcription
类别
资金
- Biotechnology and Biological Sciences Research Council [BB/C510359/1] Funding Source: researchfish
We used electron spectroscopic imaging to map nucleoplasmic transcription sites in human cells at unprecedented resolution. HeLa cells were permeabilised, nascent transcripts were extended in BrUTP by similar to 40 nucleotides and the resulting BrRNA immunolabelled with gold particles before structures were viewed. Nascent RNA is almost invariably associated with polymorphic and nitrogen-rich (but phosphorus-poor) structures with a diameter of similar to 87 nm and mass of 10 MDa (calculated by reference to nucleosomes with known numbers of phosphorus and nitrogen atoms). Structures with similar atomic signatures and diameters were observed using correlative microscopy and in unpermeabilised cells. Our results are consistent with RNA synthesis occurring on the surface of these huge protein-rich transcription factories.
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