期刊
JOURNAL OF CELL SCIENCE
卷 121, 期 6, 页码 784-795出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.021006
关键词
thrombospondins; extracellular matrix; Integrins; l-type; lectin; red fluorescent protein
类别
资金
- MRC [G0400808] Funding Source: UKRI
- Medical Research Council [G0400808] Funding Source: researchfish
- Medical Research Council [G0400808] Funding Source: Medline
- NIGMS NIH HHS [GM068073] Funding Source: Medline
- Wellcome Trust [054334] Funding Source: Medline
Thrombospondins (TSPs) are an evolutionarily ancient family of extracellular calcium-binding glycoproteins. The five mammalian TSPs collectively have important roles in angiogenesis and vascular biology, synaptogenesis, wound repair and connective tissue organisation. Their complex functions relate to the multiple postsecretion fates of TSPs that can involve endocytic uptake, proteolysis or retention within the extracellular matrix (ECM). Surprisingly, the molecular and cellular mechanisms by which TSPs become retained within the ECM are poorly understood. We hypothesised that the highly conserved TSP C-terminal domain mediates ECM retention. We report that ECM incorporation as insoluble punctate deposits is an evolutionarily conserved property of TSPs. ECM retention of TSP1 is mediated by the C-terminal region in trimeric form, and not by C-terminal monomer or trimers of the N-terminal domain or type 1 repeats. Using a novel mRFP-tagged TSP1 C-terminal trimer, we demonstrate that ECM retention involves the RGD site and a novel site in the L-lectin domain with structural similarity to the ligand-binding site of cargo transport proteins. CD47 and beta 1 integrins are dispensable for ECM retention, but beta 1 integrins enhance activity. These novel data advance concepts of the molecular processes that lead to ECM retention of TSP1.
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