期刊
JOURNAL OF CELL SCIENCE
卷 121, 期 21, 页码 3553-3560出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.033308
关键词
TLC1; Tgs1; Telomere
类别
资金
- Max-Planck-Society
- University of Duisburg-Essen
- Jurgen Manchot foundation
Telomerase in Saccharomyces cerevisiae consists of three protein subunits and the RNA moiety TLC1, which together ensure the complete replication of chromosome ends. TLC1 shares several features with snRNA, among them the presence of a trimethylguanosine (m(3)G) cap structure at the 5' end of the RNA. Here, we report that the yeast snRNA and snoRNA methyltransferase Tgs1 is responsible for TLC1 m(3)G cap formation. The absence of Tgs1 caused changes in telomere length and structure, improved telomeric silencing and stabilized telomeric recombination. Genetic analyses implicated a role for the TLC1 m(3)G cap in the coordination between telomerase and DNA polymerase for end replication. Furthermore, tgs1 Delta cells displayed a shortened replicative lifespan, suggesting that the loss of the m(3)G cap of TLC1 causes premature aging.
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