期刊
JOURNAL OF CELL SCIENCE
卷 121, 期 17, 页码 2904-2912出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.032417
关键词
wound healing; re-epithelialization; keratinocyte; Dapper2; TGF beta
类别
资金
- National Basic Research Program of China [2005CB522500, 2006BAI23B01-3, 2006CB943401]
- National Science Foundation of China [90208002, 30221003, 30430350]
- National High-Tech Research and Development Program [2006AA02Z168]
- [Z0006303041231]
Members of the Dapper (Dpr)/Dact protein family are involved in the regulation of distinct signaling pathways, including TGF beta/Nodal, canonical and noncanonical Wnt pathways. Three Dpr genes, Dpr1, Dpr2 and Dpr3, are expressed in mouse embryos and in many adult tissues; however, their in vivo functions have not been reported. In this study, we generated Dpr2-deficient mice using a gene-knockout approach. Homozygous Dpr2 knockout (Dpr2(-/-)) embryos developed normally and postnatal Dpr2(-/-) mice grew to adulthood without obvious morphological or behavioral defects. We found that Dpr2 was expressed highly in epidermal keratinocytes and in hair follicles of adult mice, and that Dpr2 deficiency resulted in accelerated re-epithelialization during cutaneous wound healing. Furthermore, we demonstrated that loss of Dpr2 function enhanced the responses of keratinocytes to TGF beta stimulation, and that TGF beta signals promoted adhesion to fibronectin and migration of keratinocytes, by regulating the expression of specific integrin genes. Thus, Dpr2 plays an inhibitory role in the re-epithelialization of adult skin wounds by attenuating TGF beta signaling.
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