期刊
JOURNAL OF CELL SCIENCE
卷 121, 期 5, 页码 627-633出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.019513
关键词
near-field scanning optical microscopy (NSOM); Interleukin receptors IL2R; IL15R; single-molecule detection; nanometer-scale membrane organization
类别
资金
- ICREA Funding Source: Custom
Interleukin 2 and interleukin 15 (IL2 and IL15, respectively) provide quite distinct contributions to T-cell-mediated immunity, despite having similar receptor composition and signaling machinery. As most of the proposed mechanisms underlying this apparent paradox attribute key significance to the individual alpha-chains of IL2 and IL15 receptors, we investigated the spatial organization of the receptors IL2R alpha and IL15R alpha at the nanometer scale expressed on a human CD4(+) leukemia T cell line using single-molecule-sensitive near-field scanning optical microscopy (NSOM). In agreement with previous findings, we here confirm clustering of IL2R alpha and IL15R alpha at the submicron scale. In addition to clustering, our single-molecule data reveal that a non-negligible percentage of the receptors are organized as monomers. Only a minor fraction of IL2R alpha molecules reside outside the clustered domains, whereas similar to 30% of IL15R alpha molecules organize as monomers or small clusters, excluded from the main domain regions. Interestingly, we also found that the packing densities per unit area of both IL2R alpha and IL15R alpha domains remained constant, suggesting a 'building block' type of assembly involving repeated structures and composition. Finally, dual-color NSOM demonstrated co-clustering of the two alpha-chains. Our results should aid understanding the action of the IL2R-IL15R system in T cell function and also might contribute to the more rationale design of IL2R- or IL15R-targeted immunotherapy agents for treating human leukemia.
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