期刊
JOURNAL OF CELL BIOLOGY
卷 207, 期 1, 页码 123-137出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201406140
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资金
- National Science Foundation [MCB1140033]
- American Heart Association [11GRNT7860023]
- National Institutes of Health/National Heart, Lung, and Blood Institute grant [R01HI107493]
Airway multiciliated epithelial cells play crucial roles in the mucosal defense system, but their differentiation process remains poorly understood. Mice lacking the basal body component Chibby (Cby) exhibit impaired mucociliary transport caused by defective ciliogenesis, resulting in chronic airway infection. In this paper, using primary cultures of mouse tracheal epithelial cells, we show that Cby facilitates basal body docking to the apical cell membrane through proper formation of ciliary vesicles at the distal appendage during the early stages of ciliogenesis. Cby is recruited to the distal appendages of centrioles via physical interaction with the distal appendage protein CEP164. Cby then associates with the membrane trafficking machinery component Rabin8, a guanine nucleotide exchange factor for the small guanosine triphosphatase Rab8, to promote recruitment of Rab8 and efficient assembly of ciliary vesicles. Thus, our study identifies Cby as a key regulator of ciliary vesicle formation and basal body docking during the differentiation of airway ciliated cells.
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