期刊
JOURNAL OF CELL BIOLOGY
卷 207, 期 5, 页码 615-626出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201404127
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资金
- Wellcome Trust PhD studentship [089693/Z/09/Z]
- Medical Research Council PhD studentship award
- Biotechnology and Biological Sciences Research Council [BB/C520404/1]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior PhD program
- Royal Society of Edinburgh/Scottish Government Personal Research Fellowship
- Royal Society Wolfson Research Merit Award
- Wellcome Trust Strategic Award [083524/Z/07/Z]
- Medical Research Council Next Generation Optical Microscopy award [MR/K015869/1]
- MRC [MC_UP_A500_1020, MR/K015869/1, G1100713, MC_UU_12016/5] Funding Source: UKRI
- Wellcome Trust [083524/Z/07/Z, 089693/Z/09/Z] Funding Source: Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BB/C520404/1] Funding Source: researchfish
- Medical Research Council [MC_UU_12016/5, MR/K015869/1, MC_UP_A500_1020, G1100713, 1324778] Funding Source: researchfish
Pathogenic bacteria adapt to their environment and manipulate the biochemistry of hosts by secretion of effector molecules. Serratia marcescens is an opportunistic pathogen associated with healthcare-acquired infections and is a prolific secretor of proteins, including three chitinases (ChiA, ChiB, and ChiC) and a chitin binding protein (Cbp21). In this work, genetic, biochemical, and proteomic approaches identified genes that were required for secretion of all three chitinases and Cbp21. A genetic screen identified a holin-like protein (ChiW) and a putative L-alanyl-D-glutamate endopeptidase (ChiX), and subsequent biochemical analyses established that both were required for nonlytic secretion of the entire chitinolytic machinery, with chitinase secretion being blocked at a late stage in the mutants. In addition, live-cell imaging experiments demonstrated bimodal and coordinated expression of chiX and chiA and revealed that cells expressing chiA remained viable. It is proposed that ChiW and ChiX operate in tandem as components of a protein secretion system used by gram-negative bacteria.
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