期刊
JOURNAL OF CELL BIOLOGY
卷 205, 期 6, 页码 791-799出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201401049
关键词
-
类别
资金
- Institut Pasteur
- Centre National de la Recherche Scientifique
- Schlumberger Foundation for Education and Research
- Fondation pour la Recherche Medicale (Equipe FRM) [DEQ20120323707]
- Fondation Association pour la Recherche sur le Cancer
- European Young Investigator award
- Institut National de la Sante et de la Recherche Medicale
- European Molecular Biology Organization Young Investigator Program
- Institut National du Cancer [2011-1-PL BIO-11-IC]
- Marie Curie postdoctoral fellowship
- Investissements d'Avenir [ANR-10-LABX-54 MEMO LIFE, ANR-11-IDEX-0001-02 PSL]
Mitotic spindle orientation relies on a complex dialog between the spindle microtubules and the cell cortex, in which F-actin has been recently implicated. Here, we report that the membrane actin linkers ezrin/radixin/moesin (ERMs) are strongly and directly activated by the Ste20-like kinase at mitotic entry in mammalian cells. Using microfabricated adhesive substrates to control the axis of cell division, we found that the activation of ERMs plays a key role in guiding the orientation of the mitotic spindle. Accordingly, impairing ERM activation in apical progenitors of the mouse embryonic neocortex severely disturbed spindle orientation in vivo. At the molecular level, ERM activation promotes the polarized association at the mitotic cortex of leucineglycine-asparagine repeat protein (LGN) and nuclear mitotic apparatus (NuMA) protein, iwo essential factors for spindle orientation. We propose that activated ERMs, together with Gai, are critical for the correct localization of LGN NuMA force generator complexes and hence for proper spindle orientation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据