期刊
JOURNAL OF CELL BIOLOGY
卷 205, 期 5, 页码 663-675出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201311050
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资金
- Developmental Fund Trustee Grant at Cincinnati Children's Hospital Medical Center
- Basil O'Connor Starter Scholar Award from the March of Dimes Foundation
- National Cancer Institute (National Institutes of Health) Intramural Research Program
- National Institutes of Health [HL085587, GM098605]
During meiosis, DNA damage response (DDR) proteins induce transcriptional silencing of unsynapsed chromatin, including the constitutively unsynapsed XY chromosomes in males. DDR proteins are also implicated in double strand break repair during meiotic recombination. Here, we address the function of the breast cancer susceptibility gene Brca1 in meiotic silencing and recombination in mice. Unlike in somatic cells, in which homologous recombination defects of Brca1 mutants are rescued by 53bp1 deletion, the absence of 53BP1 did not rescue the meiotic failure seen in Brca1 mutant males. Further, BRCA1 promotes amplification and spreading of DDR components, including AIR and TOPBP1, along XY chromosome axes and promotes establishment of pericentric heterochromatin on the X chromosome. We propose that BRCA1-dependent establishment of X-pericentric heterochromatin is critical for XY body morphogenesis and subsequent meiotic progression. In contrast, BRCA1 plays a relatively minor role in meiotic recombination, and female Brca1 mutants are fertile. We infer that the major meiotic role of BRCA1 is to promote the dramatic chromatin changes required for formation and function of the XY body.
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