期刊
JOURNAL OF CELL BIOLOGY
卷 201, 期 1, 页码 145-163出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201210019
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资金
- Canadian Cancer Society Research Institute [A020254]
- National Institutes of Health [1R01HG005853-01]
- Canadian Institutes of Health Research [MOP-102629, MOP-97939, MOP81268]
- Ontario Research Fund [GL2-01-22]
Like ubiquitin, the small ubiquitin-related modifier (SUMO) proteins can form oligomeric chains, but the biological functions of these superstructures are not well understood. Here, we created mutant yeast strains unable to synthesize SUMO chains (smt3(allR)) and subjected them to high-content microscopic screening, synthetic genetic array (SGA) analysis, and high-density transcript profiling to perform the first global analysis of SUMO chain function. This comprehensive assessment identified 144 proteins with altered localization or intensity in smt3(allR) cells, 149 synthetic genetic interactions, and 225 mRNA transcripts (primarily consisting of stress-and nutrient-response genes) that displayed a >1.5-fold increase in expression levels. This information-rich resource strongly implicates SUMO chains in the regulation of chromatin. Indeed, using several different approaches, we demonstrate that SUMO chains are required for the maintenance of normal higher-order chromatin structure and transcriptional repression of environmental stress response genes in budding yeast.
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