期刊
JOURNAL OF CELL BIOLOGY
卷 196, 期 4, 页码 513-527出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201107133
关键词
-
类别
资金
- Israel Science Foundation [308/09]
- Minerva Foundation
- German Federal Ministry of Education and Research
- Israel Cancer Research Fund
- Israel Cancer Association
- Yeda-Sela Center for Basic Research
- M.D. Morass Institute for Cancer Research
- Y. Leon Benoziyo Institute for Molecular Medicine
- Jeanne and Joseph Nissim Center for Life Sciences Research
Essentially, all metazoan cells can undergo apoptosis, but some cells are more sensitive than others to apoptotic stimuli. To date, it is unclear what determines the apoptotic potential of the cell. We set up an in vivo system for monitoring and comparing the activity levels of the two main effector caspases in Drosophila melanogaster, Drice and Dcp-1. Both caspases were activated by the apoptosome after irradiation. However, whereas each caspase alone could induce apoptosis, Drice was a more effective inducer of apoptosis than Dcp-1, which instead had a role in establishing the rate of cell death. These functional differences are attributed to their intrinsic properties rather than merely their tissue specificities. Significantly, the levels of the procaspases are directly proportional to their activity levels and play a key role in determining the cell's sensitivity to apoptosis. Finally, we provide evidence for the existence of a cellular execution threshold of caspase activity, which must be reached to induce apoptosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据