期刊
JOURNAL OF CELL BIOLOGY
卷 199, 期 3, 页码 497-512出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201201091
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资金
- National Institutes of Health [R01AI44902, R01HL65333]
- American Heart Association
- National Natural Science Foundation of China [31070827]
- Chinese Academy of Sciences
As adhesion molecules, integrins connect a cell to its environment and transduce signals across the membrane. Their different functional states correspond to distinct conformations. Using a biomembrane force probe, we observed real-time reversible switches between bent and extended conformations of a single integrin, alpha(L)beta(2), on the surface of a living cell by measuring its nanometer-scale headpiece displacements, bending and unbending frequencies, and molecular stiffness changes. We determined the stabilities of these conformations, their dynamic equilibrium, speeds and rates of conformational changes, and the impact of divalent cations and tensile forces. We quantified how initial and subsequent conformations of alpha(L)beta(2) regulate the force-dependent kinetics of dissociation from intercellular adhesion molecule 1. Our findings provide new insights into how integrins function as nanomachines to precisely control cell adhesion and signaling.
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