期刊
JOURNAL OF CELL BIOLOGY
卷 197, 期 1, 页码 45-57出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201112145
关键词
-
类别
资金
- Division of Intramural Research
- National Institute of Allergy and Infectious Diseases
- Ligue National Contre le Cancer
- Human Frontier Science Program
- European Network of Excellence DC-THERA
Whether protein translation occurs in the nucleus is contentious. To address this question, we developed the ribopuromycylation method (RPM), which visualizes translation in cells via standard immunofluorescence microscopy. The RPM is based on ribosome-catalyzed puromycylation of nascent chains immobilized on ribosomes by antibiotic chain elongation inhibitors followed by detection of puromycylated ribosome-bound nascent chains with a puromycin (PMY)-specific monoclonal antibody in fixed and permeabilized cells. The RPM correlates localized translation with myriad processes in cells and can be applied to any cell whose translation is sensitive to PMY. In this paper, we use the RPM to provide evidence for translation in the nucleoplasm and nucleolus, which is regulated by infectious and chemical stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据