期刊
JOURNAL OF CELL BIOLOGY
卷 198, 期 4, 页码 711-730出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201203099
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资金
- CNRS
- FRM-BNP-Paribas
- ARC [4979]
- [ANR-09-BLAN-0299-01]
Membrane lipids have been implicated in many critical cellular processes, yet little is known about the role of asymmetric lipid distribution in cell morphogenesis. The phosphoinositide bis-phosphate PI(4,5)P-2 is essential for polarized growth in a range of organisms. Although an asymmetric distribution of this phospholipid has been observed in some cells, long-range gradients of PI(4,5)P-2 have not been observed. Here, we show that in the human pathogenic fungus Candida albicans a steep, long-range gradient of PI(4,5)P-2 occurs concomitant with emergence of the hyphal filament. Both sufficient PI(4) P synthesis and the actin cytoskeleton are necessary for this steep PI(4,5)P-2 gradient. In contrast, neither microtubules nor asymmetrically localized mRNAs are critical. Our results indicate that a gradient of PI(4,5)P-2, crucial for filamentous growth, is generated and maintained by the filament tip-localized PI(4) P-5-kinase Mss4 and clearing of this lipid at the back of the cell. Furthermore, we propose that slow membrane diffusion of PI(4,5)P-2 contributes to the maintenance of such a gradient.
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