期刊
JOURNAL OF CELL BIOLOGY
卷 197, 期 5, 页码 595-604出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201110047
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资金
- Deutsche Forschungsgemeinschaft [Sonderforschungsbereich 746]
- Excellence Initiative of the German Federal and State Governments (Centre for Biological Signalling Studies
- GSC-4 Spemann Graduate School
- Bundesministerium fur Bildung und Forschung (Dynamo)
- Landesforschungspreis Baden-Wurttemberg
- Gottfried Wilhelm Leibniz Program
- Boehringer Ingelheim
- [Graduiertenkolleg 845]
Many mitochondrial proteins are synthesized with N-terminal presequences in the cytosol. The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor. The TIM23 complex consists of three core components and Tim21, which interacts with the translocase of the outer membrane (TOM) and the respiratory chain. We have identified a new subunit of the TIM23 complex, the inner membrane protein Mgr2. Mitochondria lacking Mgr2 were deficient in the Tim21-containing sorting form of the TIM23 complex. Mgr2 was required for binding of Tim21 to TIM23(CORE), revealing a binding chain of TIM23(CORE)-Mgr2/Tim21-respiratory chain. Mgr2-deficient yeast cells were defective in growth at elevated temperature, and the mitochondria were impaired in TOM-TIM23 coupling and the import of presequence-carrying preproteins. We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import.
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