期刊
JOURNAL OF CELL BIOLOGY
卷 196, 期 6, 页码 775-788出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201201038
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资金
- Swiss National Foundation [31003A_ 124902/1]
- German Research Foundation [SFB 780, A4]
- Alexander von Humboldt Foundation
- Grants-in-Aid for Scientific Research [22500305] Funding Source: KAKEN
- Swiss National Science Foundation (SNF) [31003A_124902] Funding Source: Swiss National Science Foundation (SNF)
Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were similar to 10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.
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