期刊
JOURNAL OF CELL BIOLOGY
卷 194, 期 1, 页码 88-102出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201011118
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- Agence Nationale de la Recherche
- Fondation pour la Recherche Medicale
- Centre National de la Recherche Scientifique
- Ministere de l'Enseignement Superieur et de la Recherche
Membrane curvature sensors have diverse structures and chemistries, suggesting that they might have the intrinsic capacity to discriminate between different types of vesicles in cells. In this paper, we compare the in vitro and in vivo membrane-binding properties of two curvature sensors that form very different amphipathic helices: the amphipathic lipid-packing sensor (ALPS) motif of a Golgi vesicle tether and the synaptic vesicle protein alpha-synuclein, a causative agent of Parkinson's disease. We demonstrate the mechanism by which alpha-synuclein senses membrane curvature. Unlike ALPS motifs, alpha-synuclein has a poorly developed hydrophobic face, and this feature explains its dual sensitivity to negatively charged lipids and to membrane curvature. When expressed in yeast cells, these two curvature sensors were targeted to different classes of vesicles, those of the early secretory pathway for ALPS motifs and to negatively charged endocytic/post-Golgi vesicles in the case of alpha-synuclein. Through structures with complementary chemistries, alpha-synuclein and ALPS motifs target distinct vesicles in cells by direct interaction with different lipid environments.
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