期刊
JOURNAL OF CELL BIOLOGY
卷 185, 期 1, 页码 35-42出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200811106
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资金
- National Institutes of Health [AI065858, AI033600, AR0514732]
Stress-induced phosphorylation of eIF alpha inhibits global protein synthesis to conserve energy for repair of stress-induced damage. Stress-induced translational arrest is observed in cells expressing a non-phosphorylatable eIF2 alpha mutant (S51A), which indicates the existence of an alternative pathway of translational control. In this paper, we show that arsenite, heat shock, or ultraviolet irradiation promotes transfer RNA (tRNA) cleavage and accumulation of tRNA-derived, stress-induced small RNAs (tiRNAs). We show that angiogenin, a secreted ribonuclease, is required for stress-induced production of tiRNAs. Knockdown of angiogenin, but not related ribonucleases, inhibits arsenite-induced tiRNA production and translational arrest. In contrast, knockdown of the angiogenin inhibitor RNH1 enhances tiRNA production and promotes arsenite-induced translational arrest. Moreover, recombinant angiogenin, but not RNase 4 or RNase A, induces tiRNA production and inhibits protein synthesis in the absence of exogenous stress. Finally, transfection of angiogenin-induced tiRNAs promotes phospho-eIF2 alpha-independent translational arrest. Our results introduce angiogenin and tiRNAs as components of a phospho-eIF2 alpha-independent stress response program.
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