期刊
JOURNAL OF CELL BIOLOGY
卷 186, 期 6, 页码 863-880出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200904111
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资金
- Swiss National Science Foundation
- Swiss Federal Institute of Technology
Before entering translation, preribosomal particles undergo sequential late maturation steps. In the case of pre-60S particles, these steps involve the release of shuttling maturation factors and transport receptors. In this study, we report a new maturation step in the 60S biogenesis pathway in budding yeast. We show that efficient release of the nucleolar/nuclear ribosomal-like protein Mrt4 (homologous to the acidic ribosomal P-protein Rpp0) from pre-60S particles requires the highly conserved protein Yvh1, which associates only with late pre-60S particles. Cell biological and biochemical analyses reveal that Mrt4 fails to dissociate from late pre-60S particles in yvh1 Delta cells, inducing a delay in nuclear preribosomal RNA processing and a pre-60S export defect in yvh1 Delta cells. Moreover, we have isolated gain of function alleles of Mrt4 that specifically bypass the requirement for Yvh1 and rescue all yvh1 Delta-associated phenotypes. Together, our data suggest that Yvh1-mediated release of Mrt4 precedes cytoplasmic loading of Rpp0 on pre-60S particles and is an obligatory late step toward construction of translation-competent 60S subunits.
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