期刊
JOURNAL OF CELL BIOLOGY
卷 183, 期 6, 页码 1075-1088出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200806035
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资金
- Deutsche Forschungsgemeinschaft [1108, TA 36/18-1]
- Czech Republic [204/06/0009, 204/08/J024, 204/07/0133]
- National Institutes of Health (National Institute of Diabetes and Digestive and Kidney Diseases) [1RO1DK079109-01]
- National Science Foundation 2010 [MCB-0618402]
In this study, we investigate whether the stable segregation of proteins and lipids within the yeast plasma membrane serves a particular biological function. We show that 21 proteins cluster within or associate with the ergosterol-rich membrane compartment of Can1 (MCC). However, proteins of the endocytic machinery are excluded from MCC. In a screen, we identified 28 genes affecting MCC appearance and found that genes involved in lipid biosynthesis and vesicle transport are significantly over-represented. Deletion of Pil1, a component of eisosomes, or of Nce102, an integral membrane protein of MCC, results in the dissipation of all MCC markers. These deletion mutants also show accelerated endocytosis of MCC-resident permeases Can1 and Fur4. Our data suggest that release from MCC makes these proteins accessible to the endocytic machinery. Addition of arginine to wild-type cells leads to a similar redistribution and increased turnover of Can1. Thus, MCC represents a protective area within the plasma membrane to control turnover of transport proteins.
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