期刊
JOURNAL OF CELL BIOLOGY
卷 181, 期 6, 页码 1027-1039出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200803150
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- NINDS NIH HHS [NS29996, NS050674, R01 NS050674, R01 NS029996] Funding Source: Medline
Cytoplasmic dynein is the multisubunit motor protein for retrograde movement of diverse cargoes to microtubule minus ends. Here, we investigate the function of dynein variants, defined by different intermediate chain (IC) isoforms, by expressing fluorescent ICs in neuronal cells. Green fluorescent protein (GFP)-IC incorporates into functional dynein complexes that copurify with membranous organelles. In living PC12 cell neurites, GFP-dynein puncta travel in both the anterograde and retrograde directions. In cultured hippocampal neurons, neurotrophin receptor tyrosine kinase B (TrkB) signaling endosomes are transported by cytoplasmic dynein containing the neuron-specific IC-1B isoform and not by dynein containing the ubiquitous IC-2C isoform. Similarly, organelles containing TrkB isolated from brain by immuno-affinity puri. cation also contain dynein with IC-1 but not IC-2 isoforms. These data demonstrate that the IC isoforms de. ne dynein populations that are selectively recruited to transport distinct cargoes.
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