期刊
JOURNAL OF CELL BIOLOGY
卷 181, 期 4, 页码 587-594出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200803027
关键词
-
类别
资金
- NIAID NIH HHS [R01 AI062427, AI062427] Funding Source: Medline
- NIGMS NIH HHS [T32 GM008347, 5T32GM008347, R37 GM032238, GM068676, GM32238, GM24364, R01 GM024364, R01 GM068676, R01 GM032238-23, R01 GM032238, R37 GM024364] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Point and regional centromeres specify a unique site on each chromosome for kinetochore assembly. The point centromere in budding yeast is a unique 150-bp DNA sequence, which supports a kinetochore with only one microtubule attachment. In contrast, regional centromeres are complex in architecture, can be up to 5 Mb in length, and typically support many kinetochore-microtubule attachments. We used quantitative fluorescence microscopy to count the number of core structural kinetochore protein complexes at the regional centromeres in fission yeast and Candida albicans We find that the number of CENP-A nucleosomes at these centromeres reflects the number of kinetochore-microtubule attachments instead of their length. The numbers of kinetochore protein complexes per microtubule attachment are nearly identical to the numbers in a budding yeast kinetochore. These findings reveal that kinetochores with multiple microtubule attachments are mainly built by repeating a conserved structural subunit that is equivalent to a single microtubule attachment site.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据