期刊
JOURNAL OF CELL BIOLOGY
卷 180, 期 1, 页码 91-100出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200710164
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- NCRR NIH HHS [P41 RR000592, RR-00592] Funding Source: Medline
- NIBIB NIH HHS [F31 EB005568, EB005568] Funding Source: Medline
- NIGMS NIH HHS [GM-60678, R37 GM032238, GM-24364, R01 GM032238-21, R37 GM024364, R01 GM071522, GM-32238, R01 GM024364, R01 GM060678, R01 GM032238, GM-071522] Funding Source: Medline
In budding yeast, the mitotic spindle is comprised of 32 kinetochore microtubules (kMTs) and similar to 8 interpolar MTs (ipMTs). Upon anaphase onset, kMTs shorten to the pole, whereas ipMTs increase in length. Overlapping MTs are responsible for the maintenance of spindle integrity during anaphase. To dissect the requirements for anaphase spindle stability, we introduced a conditionally functional dicentric chromosome into yeast. When centromeres from the same sister chromatid attach to opposite poles, anaphase spindle elongation is delayed and a DNA breakage-fusion-bridge cycle ensues that is dependent on DNA repair proteins. We find that cell survival after dicentric chromosome activation requires the MT-binding proteins Kar3p, Bim1p, and Ase1p. In their absence, anaphase spindles are prone to collapse and buckle in the presence of a dicentric chromosome. Our analysis reveals the importance of Bim1p in maintaining a stable ipMT overlap zone by promoting polymerization of ipMTs during anaphase, whereas Kar3p contributes to spindle stability by cross-linking spindle MTs.
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