期刊
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
卷 63, 期 5, 页码 461-465出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/FJC.0000000000000060
关键词
testosterone; human smooth muscle cells; cyclic nucleotide-gated channel; vasorelaxation
资金
- Fundacao para a Ciencia e a Tecnologia (Portugal) [PEst-C/SAU/UI0709/2011]
- COMPETE program
We recently observed in human umbilical artery smooth muscle cells that testosterone activates protein kinase G and stimulates large-conductance Ca2+ activated (BKCa) and voltage sensitive (K-V) potassium channels. In the same work, we also show that atrial natriuretic peptide (ANP), an activator of particulate guanylate cyclase (pGC), stimulates the activity of BKCa and K-V channels because of protein kinase G activation. The aim of this work was to prove that the relaxant effects of testosterone are also because of the increase of cGMP because of activation of the pGC. Subsarcolemmal cGMP signals were monitored in single cells by recording the cGMP-gated current (I-CNG) in human umbilical artery smooth muscle cells expressing the wild-type rat olfactory cyclic nucleotide-gated (CNG) channel. Sodium nitroprusside (10 and 100 mu M), ANP (0.1 and 1 mu M), or testosterone (0.1, 1, and 10 mu M) induced activation of I-CNG. This activation induced by testosterone and ANP is bigger than that elicited by sodium nitroprusside. In summary, our study reveals that testosterone and ANP activate the pGC and induce vasorelaxation of human umbilical artery.
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