期刊
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
卷 60, 期 6, 页码 553-560出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/FJC.0b013e31827303fa
关键词
alpha-2 adrenergic receptors; clonidine; ischemia-reperfusion; interleukin; neutrophils; platelets; endothelium
资金
- Department of Anaesthesiology of Centre Hospitalier Universitaire UCL Mont-Godinne, 1 Avenue Dr Therasse, 5530, Yvoir, Belgium
- Department of Pharmacy, Facultes Universitaires Notre-Dame de la Paix, University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium
Ischemia-reperfusion disturbs endothelial physiology and generates a proinflammatory state. Animal studies showed that clonidine administered prior hypoxia improves posthypoxic endothelial function. To investigate this effect in human, we have assessed the postischemic endothelium function and the proinflammatory state in healthy volunteers with and without clonidine. Seven volunteers were included. Each subject underwent the experimental protocol (15 minutes nondominant forearm ischemia) with and without clonidine. Endothelial function was assessed by flow-mediated dilatation (FMD) in the brachial artery before ischemia (FMDPI), immediately after ischemia (FMDIAI), and 15 minutes after ischemia (FMD15AI). Neutrophil (CD11b/CD18) and platelet (CD42b) activations were measured by flow cytometry during reperfusion in blood samples from ischemic (local) and nonischemic (systemic) forearms. Proinflammatory state was assessed by serum concentration of interleukin (IL)-1 beta and -6. Clonidine does not influence baseline FMD (P = 0.118) but improves FMDIAI (P = 0.018) and FMD15AI (P = 0.018). It increases platelet activation in systemic circulation (P = 0.003) during reperfusion but not in local circulation (P = 0.086). Clonidine increases neutrophil activation in local circulation (P = 0.001) but not in systemic circulation (P = 0.642). In local circulation, clonidine decreases IL-6 (P = 0.044) but does not influence IL-1 beta (P = 0.113). By contrast, it decreases both IL-6 (P = 0.026) and IL-1 beta (P = 0.027) concentrations in systemic circulation. In conclusion, clonidine improves endothelial function and modulates inflammation during reperfusion.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据