Rhodococcus agglutinans sp nov., an actinobacterium isolated from a soil sample

A Gram-positive, aerobic, non-motile and non-spore forming strain, designated CFH S0262(T), was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. A polyphasic approach was used to study the taxonomic position of this new isolate. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain CFH S0262(T) belongs to the genus Rhodococcus and clustered with Rhodococcus soli DSD51W(T), Rhodococcus hoagii NBRC 103062(T), Rhodococcus defluvii Call(T) and Rhodococcus kunmingensis YIM 45607(T) (98.7, 98.5, 97.9 and 97.6 % similarities, respectively). Strain CFH S0262(T) could grow in the presence of NaCl (0-4 %, optimum 0-3 %), at pH 6.0-8.0 (optimum, pH 7.0) and at 10-40 A degrees C (optimum, 28 A degrees C). The predominant menaquinones of strain CFH S0262(T) were identified as MK-8 (H-2) and MK-8 (H-4). The major fatty acids (a parts per thousand yen10 %) were found to be C-16:0 and C-18:1 omega 9c. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, a glycolipid and two unidentified phospholipids. The DNA G+C content was determined to be 71.4 mol%. Based on a comparative analysis of phenotypic and genotypic characteristics, in combination with low values of DNA-DNA hybridization between strain CFH S0262(T) and its closest neighbours, it is proposed that strain CFH S0262(T) represents a novel species of the genus Rhodococcus, for which the name Rhodococcus agglutinans sp. nov., is proposed, with the type strain CFH S0262(T) (=CCTCC AB2014297(T)=KCTC 39118(T)).