期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 108, 期 2, 页码 453-459出版社
SPRINGER
DOI: 10.1007/s10482-015-0498-z
关键词
Actinoallomurus bryophytorum sp nov.; Polyphasic taxonomy; 16S rRNA gene
类别
资金
- National Outstanding Youth Foundation [31225024]
- National Natural Science Foundation of China [31471832, 31171913, 31372006]
- National Key Technology RD Program [2012BAD19B06]
- Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP)
- China Postdoctoral Science Foundation [2014M561319]
- Heilongjiang Postdoctoral Fund [LBH-Z14027]
A novel endophytic actinomycete, strain NEAU-TX1-15(T), was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15(T). Morphological and chemotaxonomic properties of strain NEAU-TX1-15(T) are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15(T) was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H-6) and MK-9(H-8). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C-16:0. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686(T). However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15(T) could also be differentiated from A. yoronensis NBRC 103686(T) and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (> 98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA-DNA hybridization value, indicated that strain NEAU-TX1-15(T) represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15(T) (=CGMCC 4.7200(T) = JCM 30340(T)).
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