期刊
NEUROCHEMICAL RESEARCH
卷 40, 期 8, 页码 1661-1670出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-015-1648-4
关键词
L-Theanine; Cadmium; Oxidative stress; Mitochondria apoptosis pathway; Apoptosis
资金
- Grants from Jiangsu perspective study [BY2013001-03]
- National Nature Science Foundation of China [31401004]
- program of Natural Science Research of Jiangsu Higher education Institution of China [14KJB180010]
l-Theanine is an amino acid derivative from green tea. The present work was aimed at the effect of l-theanine on neuron-like rat pheochromocytoma (PC12) cells stimulated with cadmium chloride. Treatment with l-theanine before cadmium exposure increased cell viability; the experiments of Annexin V/PI staining indicated that l-theanine inhibited cadmium-induced cell apoptosis. Meanwhile, l-theanine decreased ROS production and protected from cadmium-induced disruption of mitochondrial transmembrane potential. Compared with cadmium-treated cells, l-theanine could also decrease the ratio of Bax/Bcl-2, as well as the level of cleaved caspase-9, caspase-3 and poly(ADP-ribose) polymerase. Furthermore, l-theanine depresses cadmium-induced up regulation of phosphorylations of PI3K/Akt, MAPK ERK1/2, and JNK signaling. These data suggest that l-theanine pretreatment reduces severity of cadmium toxicity probably via antioxidant action. Therefore, it may be concluded that l-theanine could be exploited for prevention of cadmium-induced diseases.
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