期刊
JOURNAL OF CARBOHYDRATE CHEMISTRY
卷 31, 期 8, 页码 634-646出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/07328303.2012.698437
关键词
Amino sugar; Chemo-enzymatic synthesis; Glycosidase; Enzymatic transglycosylation; Dehydrative condensing agent; DMT-glycoside
资金
- Ministry of Education, Sports, Science and Technology
- [21-2850]
- Grants-in-Aid for Scientific Research [23655094, 22550105] Funding Source: KAKEN
A novel sugar adduct, 4,6-dimethoxy-1,3,5-triazin-2-yl beta-D-glucosaminide (GlcN-beta-DMT), has been prepared by the reaction of D-glucosamine (GlcN) and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-morpholinium chloride (DMT-MM). The adduct was recognized by exo-beta-D-glucosaminidase (GlcNase) from Amycolatopsis orientalis and transferred the GlcN moiety, giving rise to the corresponding glucosaminides. This chemo-enzymatic process was successfully applied to D-galactosamine (GalN). GalN-beta-DMT prepared directly from GalN and DMT-MM behaved as an efficient glycosyl donor for transfer of the GalN moiety catalyzed by the same enzyme. The introduction of the 4,6-dimethoxy-1,3,5-triazin-2-yl leaving group to the anomeric center significantly enhanced transglycosylating ability, resulting in the efficient glycosidase-catalyzed synthesis of glycosaminides.
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