期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 137, 期 11, 页码 1697-1704出版社
SPRINGER
DOI: 10.1007/s00432-011-1051-8
关键词
GKN1; Tumor suppressor; Gastric cancer; Progression; EMT
类别
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0020764]
- National Research Foundation of Korea [2010-0020764] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Gastrokine 1 (GKN1) plays an important role in the gastric mucosal defense mechanism and also acts as a functional gastric tumor suppressor. The specific aim of this study was to determine the molecular mechanisms underlying GKN1 tumor suppressor activity in the progression of gastric cancers. We examined the effect of GKN1 on epithelial-mesenchymal transition (EMT) and cell migration in GKN1-transfected and recombinant GKN1-treated AGS gastric cancer cells using in vitro wound healing, microchemotaxis, and invasion assays. In GKN1-transfected AGS cells, we observed inhibition of cell migration and invasion in wound healing, transwell and Matrigel assay. Also, GKN1-transfected and recombinant GKN1-treated AGS cells showed decreased levels of ROS and expression of phosphatidylinositol 3-kinase (PI3K)/Akt pathway proteins, concomitant with re-expression of E-cadherin and decreased expression of cytoplasmic and nuclear expression of beta-catenin, slug, snail, fibronectin, and vimentin. These data suggest that the GKN1 gene may play an important role in the progression of sporadic gastric cancers via inhibition of EMT and cancer cell migration.
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