期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 137, 期 10, 页码 1581-1585出版社
SPRINGER
DOI: 10.1007/s00432-011-1017-x
关键词
Pro-interleukin-16; Growth arrest; Malignant T cell; Lymphoblastic leukemia; Lentiviral vector
类别
资金
- National Science Foundation [0538608]
- National Institutes of Health [R01CA122737-01A2]
- Division Of Graduate Education
- Direct For Education and Human Resources [0538608] Funding Source: National Science Foundation
Purpose Pro-interleukin-16 (pro-IL-16) is the precursor to mature interleukin-16 (IL-16) protein. Previous studies have demonstrated that pro-IL-16 can function as a regulator of cell cycle. A number of human T-cell leukemia and lymphoma cell lines are pro-IL-16 deficient. Intracellular expression of pro-IL-16 causes these cell lines to become quiescent, implicating loss of pro-IL-16 as a contributory step in T-cell malignancy. Therefore, we tested whether or not reintroduction of pro-IL-16 into solid tumors in mice could halt tumor growth. Methods MOLT-4 lymphoblastic leukemia cells were stably transfected with a dsRed-tomato virus and were injected subcutaneously into NOD/SCID/gamma chain-knockout mice. Tumor growth was monitored with an in vivo imaging system. A pro-IL-16-GFP fusion virus or control GFP only virus was injected into the tumors, and mice were monitored for 1 week. Results Injection of the pro-IL-16-containing lentivirus inhibited growth of established MOLT-4 tumors in mice. Tumor explants exhibited diminished proliferative capacity. Conclusions Our data support the concept that restoration of pro-IL-16 expression in malignant T cells may have therapeutic value.
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