期刊
ACTA BIOMATERIALIA
卷 16, 期 -, 页码 136-144出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.01.017
关键词
Immunoisolation; Beta cell; Alginate; Nanoceria; Free radical
资金
- National Institutes of Health [1DP2 DK08309601]
- juvenile Diabetes Research Center Grant [4-2004-361]
- Diabetes Research Institute Foundation
Oxidative stress and the resulting radical by-products cause significant toxicity and graft loss in cellular transplantation. Here, the engineering of an auto-catalytic, antioxidant, self-renewing cerium oxide nanoparticle (CONP)-composite hydrogel is reported. This enzyme-mimetic material ubiquitously scavenges ambient free radicals, with the potential to provide indefinite antioxidant protection. The potential of this system to enhance the protection of encapsulated beta cells was evaluated. Co-incubation of CONPs free in solution with beta cells demonstrated potent cytoprotection from superoxide exposure; however, phagocytosis of the CONPs by the beta cells resulted in cytotoxicity at concentrations as low as 1 mM. When CONPs were embedded within alginate hydrogels, the composite hydrogel provided cytoprotection to encapsulated beta cells from free radical attack without cytotoxicity, even up to 10 mM. This nanocomposite hydrogel has wide applicability in cellular transplantation, with the unique advantage of localization of these potent antioxidant CONPs and their capacity for sustained, long-term scavenging. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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