期刊
JOURNAL OF BIOTECHNOLOGY
卷 190, 期 -, 页码 2-10出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2014.03.030
关键词
Cyanide biodegradation; Iron; fur; prrF; fiuA
资金
- MICINN [BIO2011-30026-C02-01]
- Gobierno de Extremadura [GR10165-CCV019, PRI07A097]
- FEDER
- Gobierno de Extremadura (Fondo Social Europeo)
The Fur protein is the main sensor of cellular iron status in bacteria. In the present study, we inactivated the fur gene of Pseudomonas pseudoalcaligenes CECT5344 and characterized the resulting mutant. Our findings provide experimental evidence that, cyanide generates an intracellular signal equivalent to that triggered by iron deprivation, as witnessed by the induction of prrF and fiuA (ferrichrome receptor) expression in the presence of cyanide. The fur mutant also displayed slow growth, especially in minimal culture medium, increased sensitivity to cyanide in LB medium and as expected, resistance to manganese ions. Moreover, the mutant exhibited enhanced iron accumulation and increased sensitivity to streptonigrin, as well as to some inducers of oxidative stress, such as paraquat and menadione, yet it remained resistant to hydrogen peroxide. Surprisingly, neither the wild type strain nor the fur mutant strain produced siderophores that could be detected using the universal CAS-agar method. (C) 2014 Elsevier B.V. All rights reserved.
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