期刊
JOURNAL OF BIOTECHNOLOGY
卷 185, 期 -, 页码 51-56出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2014.06.014
关键词
Azadirachtin; Drosophila; Growth inhibition; Digital gene expression
资金
- Fundamental Research Funds of the State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources
- National Department of Public Benefit Research Foundation of China [200903052]
Azadirachtin is a botanical insecticide that affects various biological processes. The effects of azadirachtin on the digital gene expression profile and growth inhibition in Drosophila larvae have not been investigated. In this study, we applied high-throughput sequencing technology to detect the differentially expressed genes of Drosophila larvae regulated by azadirachtin. A total of 15,322 genes were detected, and 28 genes were found to be significantly regulated by azadirachtin. Biological process and pathway analysis showed that azadirachtin affected starch and sucrose metabolism, defense response, signal transduction, instar larval or pupal development, and chemosensory behavior processes. The genes regulated by azadirachtin were mainly enriched in starch and sucrose metabolism. This study provided a general digital gene expression profile of dysregulated genes in response to azadirachtin and showed that azadirachtin provoked potent growth inhibitory effects in Drosophila larvae by regulating the genes of cuticular protein, amylase, and odorant-binding protein. Finally, we propose a potential mechanism underlying the dysregulation of the insulin/insulin-like growth factor signaling pathway by azadirachtin. (C) 2014 Elsevier B.V. All rights reserved.
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