期刊
JOURNAL OF BIOTECHNOLOGY
卷 184, 期 -, 页码 179-186出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2014.05.011
关键词
Protein scaffolds; Ultrafine fibers; 3D electrospinning; Intrinsic water stability; Adipose-derived mesenchymal stem cells; Soft tissue engineering
资金
- Agricultural Research Division at the University of Nebraska-Lincoln
- USDA Hatch Act [S-1054 (NEB 37-037)]
- Science and Technology Commission of Shanghai Municipality [12JC1400300]
- Louise Skala Fellowship
- AATCC student research grant
- UCARE award at the University of Nebraska-Lincoln
Wheat glutenin, the highly crosslinked protein from wheat, was electrospun into scaffolds with ultrafine fibers oriented randomly and evenly in three dimensions to simulate native extracellular matrices of soft tissues. The scaffolds were intrinsically water-stable without using any external crosslinkers and could support proliferation and differentiation of adipose-derived mesenchymal stem cells for soft tissue engineering. Regeneration of soft tissue favored water-stable fibrous protein scaffolds with three-dimensional arrangement and large volumes, which could be difficult to obtain via electrospinning. Wheat glutenin is an intrinsically water-stable protein due to the 2% cysteine in its amino acid composition. In this research, the disulfide crosslinks in wheat glutenin were cleaved while the backbones were preserved. The treated wheat glutenin was dissolved in aqueous solvent with an anionic surfactant and then electrospun into bulky scaffolds composed of ultrafine fibers oriented randomly in three dimensions. The scaffolds could maintain their fibrous structures after incubated in PBS for up to 35 days. In vitro study indicated that the three-dimensional wheat glutenin scaffolds well supported uniform distribution and adipogenic differentiation of adipose derived mesenchymal stem cells. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据