期刊
JOURNAL OF BIOTECHNOLOGY
卷 163, 期 2, 页码 243-249出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2012.06.036
关键词
Hydrogel; Hydrogel microspheres; Network structure; Release studies; Protein delivery; Mesh size
资金
- Deutsche Forschungsgemeinschaft (DFG) [SFB 578, D1]
Novel hydrogels based on hydroxyethyl starch modified with polyethylene glycol methacrylate (HES-P(EG)(6)MA) were developed as delivery system for the controlled release of proteins. Since the drug release behavior is supposed to be related to the pore structure of the hydrogel network the pore sizes were determined by cryo-SEM, which is a mild technique for imaging on a nanometer scale. The results showed a decreasing pore size and an increase in pore homogeneity with increasing polymer concentration. Furthermore, the mesh sizes of the hydrogels were calculated based on swelling data. Pore and mesh size were significantly different which indicates that both structures are present in the hydrogel. The resulting structural model was correlated with release data for bulk hydrogel cylinders loaded with FITC-dextran and hydrogel microspheres loaded with FITC-IgG and FITC-dextran of different molecular size. The initial release depended much on the relation between hydrodynamic diameter and pore size while the long term release of the incorporated substances was predominantly controlled by degradation of the network of the much smaller meshes. (C) 2012 Elsevier B.V. All rights reserved.
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