期刊
JOURNAL OF BIOTECHNOLOGY
卷 167, 期 4, 页码 357-364出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2013.07.023
关键词
RecA; Geraniol; Stress tolerance; SOS response; DNA repair; Escherichia coli
资金
- National Research Foundation [NRF-2010-C1AAA001-0029084]
- MEST
- Next-Generation BioGreen 21 Program, RDA, Korea [PJ0081842011]
- BK21 Program, MEST, Korea
Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli. (c) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据