期刊
JOURNAL OF BIOTECHNOLOGY
卷 164, 期 2, 页码 340-345出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2013.01.013
关键词
Solvent-free system; Enzymatic transesterification; Ethyl ferulate; Monostearin; Response surface methodology
资金
- National Natural Science Foundation of China [31101301]
- Doctoral Foundation of Henan University of Technology [2009BS022]
- Education Department of Henan Province Science and Technology Research Project [12B550001]
In this study, enzymatic transesterification of ethyl ferulate (EF) and monostearin for feruloylated lipids production was investigated. Enzyme screening and the effect of feruloyl acceptors on the transesterification were also studied. Effects of reaction variables (reaction temperatures, enzyme load, and reaction time) on the transesterification were optimized using response surface methodology (RSM). The optimum conditions were as follows: reaction temperature 74 degrees C, reaction time 23 h, and enzyme load 20% (w/w, relative to the weight of substrates). Under these conditions, EF conversion was 98.3 +/- 1.1%, and the transesterification product was consisted of 19.2 +/- 2.1% glyceryl ferulate (FG), 32.9 +/- 1.9% diferuloylated glycerols (DFG), 36.6 +/- 2.2% feruloylated monoacylglycerols (FMAG), 9.1 +/- 2.0% feruloylated diacylglycerols (FDAG), and 0.5% ferulic acid (FA). Analysis of variance (ANOVA) showed that the regression equation was adequate for predicting EF conversion. The activation energies for hydrolysis to form FG + DFG and transesterification to form FMAG + FDAG were calculated as 22.45 and 51.05 kJ/mol, respectively, based on Arrhenius law. (C) 2013 Elsevier B.V. All rights reserved.
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