期刊
JOURNAL OF BIOTECHNOLOGY
卷 166, 期 3, 页码 114-121出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2013.04.016
关键词
Agronomic traits; Brassica juncea; Herbicide tolerance; Nonexpressor of pathogenesis-related gene; Pathogen resistance; Transgenic rice
资金
- M/S. Swarna Bharat Biotechnics Pvt. Ltd. Hyderabad, India
- Department of Biotechnology, Government of India (New Delhi)
- Council of Scientific and Industrial Research, New Delhi
Brassica juncea Nonexpressor of pathogenesis-related genes 1 (BjNPR1) has been introduced into commercial indica rice varieties by Agrobacterium-mediated genetic transformation. Transgenic rice plants were regenerated from the phosphinothricin-resistant calli obtained after co-cultivation with Agrobacterium strain LBA4404 harbouring Ti plasmid pSB111-bar-BjNPR1. Molecular analyses confirmed the stable integration and expression of BjNPR1 in various transgenic rice lines. Transgenes NPR1 and bar were stably inherited and disclosed co-segregation in subsequent generations in a Mendelian fashion. Homozygous transgenic rice lines expressing BjNPR1 protein displayed enhanced resistance to rice blast, sheath blight and bacterial leaf blight diseases. Rice transformants with higher levels of NPR1 revealed notable increases in plant height, panicle length, flag-leaf length, number of seeds/panicle and seed yield/plant as compared to the untransformed plants. The overall results amply demonstrate the profound impact of BjNPR1 in imparting resistance against major pathogens of rice. The multipotent BjNPR1, as such, seems promising as a prime candidate gene to fortify crop plants with durable resistance against various pathogens. (c) 2013 Elsevier B.V. All rights reserved.
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