期刊
JOURNAL OF BIOTECHNOLOGY
卷 162, 期 1, 页码 21-27出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2012.04.018
关键词
Dunaliella; Turbidostat; Carotenoid; Fatty acid; Nitrogen starvation; Metabolism
资金
- Technology Foundation STW, Netherlands Organisation for Scientific Research [WLM.6622]
- Centre for Biosystems Genomics under Netherlands Genomics Initiative
Nitrogen availability and light intensity affect beta-carotene overproduction in the green alga Dunaliella sauna. Following a previous study on high-light stress, we here report on the effect of nitrogen depletion on the growth characteristics and beta-carotene as well as fatty acid metabolism of D. salina under a constant light regime in a turbidostat. Upon nitrogen depletion, the biomass yield on absorbed light approximately doubled, due to a transient increase in cell division rate, swelling of the cells and a linear increase of the density of the cells. Simultaneously, beta-carotene started to accumulate up to a final intracellular concentration of 1 4 mg LCV-1 (i.e. 2.7% of AFDW). This beta-carotene production accounted for 6% of the increased density of the cells, indicating that other biochemical constituents accumulated as well. Since D. sauna accumulates beta-carotene in lipid globules, we also determined the fatty acid content and composition of D. saline. The intracellular concentration of the total fatty acid pool did not change significantly during nitrogen starvation, indicating that beta-carotene and total fatty acid accumulation were unrelated, similar to what was found previously for high-light treated cells. However, for both high-light and nitrogen stress, beta-carotene accumulation negatively correlated with the degree of unsaturation of the total fatty acid pool and, within the individual fatty acids, correlated positively with oleic acid biosynthesis, suggesting that oleic acid may be a key component of the lipid-globule-localized triacylglycerols and thereby in beta-carotene accumulation. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据