期刊
JOURNAL OF BIOTECHNOLOGY
卷 157, 期 1, 页码 167-172出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2011.10.006
关键词
UP element; -35 and-10 elements; ITPG-induction
资金
- Bavarian Science Foundation
- DLR [VNB02/B03]
- MOST [Life Science-643204]
Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein production in Bacillus subtilis cells. Here, we present experimental data how to generate strong IPTG-inducible promoters by optimization of nucleotides at the conserved regions of the groESL promoter including the UP element, the -35, -15, -10 and the +1 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据