期刊
JOURNAL OF BIOTECHNOLOGY
卷 162, 期 2-3, 页码 237-245出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2012.09.019
关键词
Biogenic magnetization; Biomagnetism; Cell separation; Metabolic engineering; Superparamagnetism; Synthetic biology
资金
- Swiss National Science Foundation [31003A-126022]
Although magnetic fields and paramagnetic inorganic materials were abundant on planet earth during the entire evolution of living species the interaction of organisms with these physical forces remains a little-understood phenomenon. Interestingly, rather than being genetically encoded, organisms seem to accumulate and take advantage of inorganic nanoparticles to sense or react to magnetic fields. Using a synthetic biology-inspired approach we have genetically programmed mammalian cells to show superparamagnetic behavior. The combination of ectopic production of the human ferritin heavy chain 1 (hFTH1), engineering the cells for expression of an iron importer, the divalent metal ion transferase 1 (DMT1) and the design of an iron-loading culture medium to maximize cellular iron uptake enabled efficient iron mineralization in intracellular ferritin particles and conferred superparamagnetic behavior to the entire cell. When captured by a magnetic field the superparamagnetic cells reached attraction velocities of up to 30 mu m/s and could be efficiently separated from complex cell mixtures using standard magnetic cell separation equipment. Technology that enables magnetic separation of genetically programmed superparamagnetic cells in the absence of inorganic particles could foster novel opportunities in diagnostics and cell-based therapies. (C) 2012 Elsevier B. V. All rights reserved.
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