期刊
JOURNAL OF BIOTECHNOLOGY
卷 158, 期 4, 页码 203-210出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2011.06.025
关键词
Bioethanol; Biomass; Lignocellulose; Cell surface display
资金
- New Energy and Industrial Technology Development Organization (NEDO) [P07015]
- Ministry of Economy, Trade, and Industry (METI) of Japan
- Grants-in-Aid for Scientific Research [22780092] Funding Source: KAKEN
The cost of the lignocellulose-hydrolyzing enzymes used in the saccharification process of ethanol production from biomass accounts for a relatively high proportion of total processing costs. Cell surface engineering technology has facilitated a reduction in these costs by integrating saccharification and fermentation processes into a recombinant microbe strain expressing heterologous enzymes on the cell surface. We constructed a recombinant Saccharomyces cerevisiae that not only hydrolyzed hemicelluloses by codisplaying endoxylanase from Trichoderma reesei, beta-xylosidase from Aspergillus oryzae, and beta-glucosidase from Aspergillus aculeatus but that also assimilated xylose through the expression of xylose reductase and xylitol dehydrogenase from Pichia stipitis and xylulokinase from S. cerevisiae. The recombinant strain successfully produced ethanol from rice straw hydrolysate consisting of hemicellulosic material containing xylan, xylooligosaccharides, and cellooligosaccharides without requiring the addition of sugar-hydrolyzing enzymes or detoxication. The ethanol titer of the strain was 8.2 g/l after 72 h fermentation, which was approximately 2.5-fold higher than that of the control strain. The yield (grams of ethanol per gram of total sugars in rice straw hydrolysate consumed) was 0.41 g/g, which corresponded to 82% of the theoretical yield. The cell surface-engineered strain was thus highly effective for consolidating the process of ethanol production from hemicellulosic materials. (C) 2011 Elsevier B.V. All rights reserved.
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